Coding

Part:BBa_K2036000:Design

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-18)


CII bacteriophage lambda trascriptional activator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CII is an transcriptional activator,it can direct pRE downstream gene expression, when applied in E.coli, one should take considerations of Ftsh which could degrade CII normally in vivo.

Source

come from bacteriaphage lambda

References

Datta AB1, Roy S, Parrack P. Role of C-terminal residues in oligomerization and stability of lambda CII: implications for lysis-lysogeny decision of the phage. J Mol Biol. 2005 Jan 14;345(2):315-24.